16S ribosomal RNA gene-based metagenomics: A review
نویسندگان
چکیده
منابع مشابه
Clostridium bacteraemia characterised by 16S ribosomal RNA gene sequencing.
BACKGROUND Owing to problems in accurate species identification of the diverse genus clostridium, the epidemiology and pathogenicity of many species are not fully understood. Moreover, previous studies on clostridium bacteraemia have been limited and relied only on phenotypic species identification. AIMS To characterise the epidemiology, disease spectrum, and outcome of clostridium bacteraemi...
متن کاملGlobicatella bacteraemia identified by 16S ribosomal RNA gene sequencing.
BACKGROUND Globicatella are streptococcus-like organisms that have been rarely isolated from clinical specimens. Their epidemiology and clinical significance remain largely unknown. AIMS To describe two cases of Globicatella bacteraemia identified by 16S ribosomal RNA (rRNA) gene sequencing. METHODS Two unidentified streptococcus-like bacteria isolated from blood cultures of patients were s...
متن کاملGemella bacteraemia characterised by 16S ribosomal RNA gene sequencing.
AIMS To define epidemiology, clinical disease, and outcome of gemella bacteraemia by 16S rRNA gene sequencing. To examine the usefulness of the Vitek, API, and ATB systems in identifying two gemella species. METHODS All alpha haemolytic streptococci other than Streptococcus pneumoniae isolated from blood cultures during a six year period were identified by conventional biochemical methods, th...
متن کاملNext-generation sequencing of 16S ribosomal RNA gene amplicons.
One of the major questions in microbial ecology is "who is there?" This question can be answered using various tools, but one of the long-lasting gold standards is to sequence 16S ribosomal RNA (rRNA) gene amplicons generated by domain-level PCR reactions amplifying from genomic DNA. Traditionally, this was performed by cloning and Sanger (capillary electrophoresis) sequencing of PCR amplicons....
متن کاملThe 16S ribosomal RNA mutation database (16SMDB)
The 16S ribosomal RNA mutation database (16SMDB) provides a list of mutated positions in 16S ribosomal RNA from Escherichia coli and the identity of each alteration. Information provided for each mutation includes: (i) a brief description of the phenotype(s) associated with each mutation; (ii) whether a mutant phenotype has been detected by in vivo or in vitro methods; (iii) relevant literature...
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ژورنال
عنوان ژورنال: Biomedical Research Journal
سال: 2020
ISSN: 2349-3666
DOI: 10.4103/bmrj.bmrj_4_20